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Universal method for the determination of nonionic surfactant content in the presence of protein
Author(s) -
Wei Ziping,
Bilbulian Susanna,
Li Jingning,
Pandey Ratnesh,
O'Connor Ellen,
CasasFinet Jose,
Cash Patricia W.
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201570081
Subject(s) - ethylene glycol , nonionic surfactant , chemistry , chromatography , hydrolysis , pulmonary surfactant , peg ratio , poloxamer , polymer , organic chemistry , copolymer , biochemistry , finance , economics
J. Sep. Sci. 2015, 38, 1318–1325 DOI: 10.1002/jssc.201400766 This cover picture illustrates how multiple nonionic surfactants can be quantified using the same method as they share a common hydrolysis product. Protein samples containing nonionic surfactants are hydrolyzed and the released ethylene glycol diacetate (EGD) is quantitated by capillary gas chromatography (GC). The chromatographic peak at 8.27 minutes retention time was identified as EGD (shown on white background). The content of PEG 8000, PS‐80, and Pluronic F‐68 (formulas shown in attached image) was determined by comparing the released ethylene glycol diacetate signal to that measured from calibration standards. Principles from this approach may be applied with success to determine the PEG content in PEGylated proteins.