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Synthesis of a stationary phase based on silica modified with branched octadecyl groups by Michael addition and photoinduced thiol–yne click chemistry for the separation of basic compounds
Author(s) -
Huang Guang,
Ou Junjie,
Wang Hongwei,
Ji Yongsheng,
Wan Hao,
Zhang Zhang,
Peng Xiaojun,
Zou Hanfa
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201501403
Subject(s) - chemistry , michael reaction , acetonitrile , click chemistry , thiol , fourier transform infrared spectroscopy , silica gel , phase (matter) , raman spectroscopy , organic chemistry , chromatography , catalysis , chemical engineering , physics , optics , engineering
A novel silica‐based stationary phase with branched octadecyl groups was prepared by the sequential employment of the Michael addition reaction and photoinduced thiol–yne click chemistry with 3‐aminopropyl‐functionalized silica microspheres as the initial material. The resulting stationary phase denoted as SiO 2 ‐N(C18) 4 was characterized by elemental analysis, FTIR spectroscopy and Raman spectroscopy, demonstrating the existence of branched octadecyl groups in silica microspheres. The separations of benzene homologous compounds, acid compounds and amine analogues were conducted, demonstrating mixed‐mode separation mechanism on SiO 2 ‐N(C18) 4 . Baseline separation of basic drugs mixture was acquired with the mobile phase of acetonitrile/H 2 O (5%, v/v). SiO 2 ‐N(C18) 4 was further applied to separate Corydalis yanhusuo Wang water extracts, and more baseline separation peaks were obtained for SiO 2 ‐N(C18) 4 than those on Atlantis dC18 column. It can be expected that this new silica‐based stationary phase will exhibit great potential in the analysis of basic compounds.