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Molecularly imprinted polymer on a SiO 2 ‐coated graphene oxide surface for the fast and selective dispersive solid‐phase extraction of Carbamazepine from biological samples
Author(s) -
Khalilian Faezeh,
Ahmadian Setareh
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201501392
Subject(s) - molecularly imprinted polymer , ethylene glycol dimethacrylate , polymer , methacrylic acid , solid phase extraction , ethylene oxide , polymerization , graphene , adsorption , materials science , fourier transform infrared spectroscopy , oxide , chemistry , detection limit , analytical chemistry (journal) , chemical engineering , chromatography , copolymer , organic chemistry , selectivity , nanotechnology , engineering , catalysis
A surface carbamazepine‐imprinted polymer was grafted and synthesized on the SiO 2 /graphene oxide surface. Firstly SiO 2 was coated on synthesized graphene oxide sheet using the sol–gel technique. Prior to polymerization, the vinyl group was incorporated on to the surface of SiO 2 /graphene oxide to direct selective polymerization on the surface. Methacrylic acid, ethylene glycol dimethacrylate and ethanol were used as monomer, cross‐linker and porogen, respectively. Nonimprinted polymer was also prepared for comparison. The properties of the molecularly imprinted polymer were characterized using field‐emission scanning electron microscopy and Fourier‐transform infrared spectroscopy. The surface molecularly imprinted polymer was utilized as an adsorbent of dispersive solid‐phase extraction for separation and preconcentration of carbamazepine. The effects of the different parameters influencing the extraction efficiency, such as sample pH were investigated and optimized. The specificity of the molecular imprinted polymer over the nonimprinted polymer was examined in absence and presence of competitive drugs. The carbamazepine calibration curve showed linearity in the ranges 0.5–500 μg/L. The limits of detection and quantification under the optimized conditions were 0.1 and 0.3 μg/L, respectively. The within‐day and between‐day relative standard deviations ( n = 3) were 3.6 and 4.3%, respectively. Furthermore, the relative recoveries for spiked biological samples were above 85%.