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Classification of the medicinal plants of the genus Atractylodes using high‐performance liquid chromatography with diode array and tandem mass spectrometry detection combined with multivariate statistical analysis
Author(s) -
Cho HyunDeok,
Kim Unyong,
Suh Joon Hyuk,
Eom Han Young,
Kim Junghyun,
Lee Seul Gi,
Choi Yong Seok,
Han Sang Beom
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201501279
Subject(s) - chromatography , tandem , atractylodes , tandem mass spectrometry , mass spectrometry , high performance liquid chromatography , statistical analysis , chromatography detector , chemistry , electrospray , multivariate analysis , analytical chemistry (journal) , materials science , computer science , mathematics , medicine , alternative medicine , traditional chinese medicine , pathology , statistics , machine learning , composite material
Analytical methods using high‐performance liquid chromatography with diode array and tandem mass spectrometry detection were developed for the discrimination of the rhizomes of four Atractylodes medicinal plants: A. japonica, A. macrocephala, A. chinensis , and A. lancea . A quantitative study was performed, selecting five bioactive components, including atractylenolide I, II, III, eudesma‐4(14),7(11)‐dien‐8‐one and atractylodin, on twenty‐six Atractylodes samples of various origins. Sample extraction was optimized to sonication with 80% methanol for 40 min at room temperature. High‐performance liquid chromatography with diode array detection was established using a C 18 column with a water/acetonitrile gradient system at a flow rate of 1.0 mL/min, and the detection wavelength was set at 236 nm. Liquid chromatography with tandem mass spectrometry was applied to certify the reliability of the quantitative results. The developed methods were validated by ensuring specificity, linearity, limit of quantification, accuracy, precision, recovery, robustness, and stability. Results showed that cangzhu contained higher amounts of atractylenolide I and atractylodin than baizhu, and especially atractylodin contents showed the greatest variation between baizhu and cangzhu. Multivariate statistical analysis, such as principal component analysis and hierarchical cluster analysis, were also employed for further classification of the Atractylodes plants. The established method was suitable for quality control of the Atractylodes plants.