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Monosaccharide composition analysis of immunomodulatory polysaccharides by on‐line hollow fiber microextraction with high‐performance liquid chromatography
Author(s) -
Wang Nani,
Wang Xuping,
Huang Xiaowen,
Mao Zhujun,
Zhang Yang,
Yu Yong,
Shou Dan
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201501205
Subject(s) - monosaccharide , chromatography , derivatization , polysaccharide , chemistry , solid phase microextraction , high performance liquid chromatography , uronic acid , fiber , gas chromatography , detection limit , organic chemistry , gas chromatography–mass spectrometry , mass spectrometry
The monosaccharide compositions of functional polysaccharides are essential for structure elucidation and biological activity determination. A sensitive method based on on‐line hollow‐fiber liquid‐phase microextraction with high‐performance liquid chromatography has been established for the analysis of ten monosaccharide compositions (two uronic acids, two amino sugars and six neutral sugars) of the immunomodulatory polysaccharides. After derivatization , the sample was injected into the lumen of a hollow fiber immersed in butyl ether and separated by liquid chromatography. Under optimized conditions, the calibration curves were linear ( r ≥ 0.9996) in the range of 10–2000 μmol L −1 . The limits of detection were in the range of 0.04–1.58 μmol L −1 , and the recoveries were in the range of 92.1–99.6%, which shows that the method is applicable to the analysis of the monosaccharide composition of various polysaccharides.