Premium
Determination of donepezil in serum samples using molecularly imprinted polymer nanoparticles followed by high‐performance liquid chromatography with ultraviolet detection
Author(s) -
Khansari Mehdi Rajabnia,
Bikloo Shahrzad,
Shahreza Sara
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201501178
Subject(s) - molecularly imprinted polymer , solid phase extraction , molecular imprinting , chromatography , elution , chemistry , donepezil , extraction (chemistry) , high performance liquid chromatography , polymer , acetonitrile , selectivity , organic chemistry , medicine , dementia , disease , pathology , catalysis
A molecularly imprinted polymer designed for the selective extraction of donepezil from serum samples was synthesized using a noncovalent molecular imprinting approach. The molecularly imprinted polymer was evaluated chromatographically and then its affinity for donepezil was confirmed by solid‐phase extraction. The optimal conditions for solid‐phase extraction were provided by cartridge conditioning using acidified water purified from a Milli‐Q system, sample loading under basic aqueous conditions, clean‐up using acetonitrile, and elution with methanol/tetrahydrofuran. Desirable molecular recognition properties of the molecularly imprinted polymer led to good donepezil recoveries (90–102%). The data indicated that the imprinted polymer has a perfect selectivity and affinity for donepezil and could be used for selective extraction and analysis of donepezil in human serum.