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Reversed‐phase ion‐pair high‐performance liquid chromatography assay of polyprenyl diphosphate oligomer homologues
Author(s) -
Kozlov Vyacheslav V.,
Danilov Leonid L.
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201501098
Subject(s) - oligomer , reagent , chemistry , chromatography , phosphate , high performance liquid chromatography , ion , phase (matter) , organic chemistry
A reversed‐phase ion‐pair high‐performance liquid chromatography procedure was developed for the separation of polyprenyl diphosphate oligomer homologues obtained chemically from plant polyprenols. Tetrabutylammonium phosphate was used as the ion‐pair reagent, and the dependence of the separation quality on pH of ion‐pair reagent was investigated for the first time. The procedure is applicable for the control of commercial available polyprenyl monophosphates (the active components of veterinary drugs Phosprenyl and Gamapren) for the possible presence of polyprenyl diphosphate byproducts.