Premium
Mass spectrometric analysis of free fatty acids in infant milk powders by frozen pretreatment coupled with isotope‐labeling derivatization
Author(s) -
Zhou Tianxiao,
Leng Jiapeng,
Peng Yaoshan,
Zhang Lei,
Guo Yinlong
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201501032
Subject(s) - derivatization , chemistry , chromatography , reagent , fatty acid , infant formula , free form , mass spectrometry , food science , biochemistry , organic chemistry , computer science , computer graphics (images)
In combination with frozen pretreatment and carboxyl group derivatization, a novel workflow was developed for the determination of free fatty acids in milk powder. The workflow showed a significantly enhanced performance for comprehensive free fatty acid analysis owing to a highly efficient frozen extraction method. In addition, the advantages of the workflow also involved high sensitivity and great tolerance to a complex matrix. Characteristic fragment ions of derivatization reagents also provide clear evidence for the qualitative analysis of free fatty acids. Fourteen types of free fatty acids in a number of domestic and overseas infant milk powders have been successfully detected. The content of free fatty acids in the different samples was different, which probably indicates the diverse quality of infant milk powder. The workflow is expected to be a pragmatic tool for the analysis of free fatty acids in intricate matrices.