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Recent developments and applications of capillary and microchip electrophoresis in proteomic and peptidomic analyses
Author(s) -
Štěpánová Sille,
Kašička Václav
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500973
Subject(s) - electromigration , capillary electrophoresis , proteomics , mass spectrometry , capillary action , chemistry , chromatography , capillary electrophoresis–mass spectrometry , nanotechnology , materials science , electrospray ionization , biochemistry , composite material , gene
This review presents recent developments and applications of capillary and microchip electromigration methods in proteomics and peptidomics. Sample preparation methods as well as instrumental innovations in the coupling of these advanced electromigration methods with mass spectrometry detection employed in proteomic and peptidomic analyses are presented. Interesting applications of various capillary electromigration methods in bottom‐up as well as top‐down proteomics, including investigation of post‐translational modifications of proteins are described. In addition, several examples of the use of capillary electromigration methods combined with mass spectrometry detection in clinical proteomics and peptidomics are demonstrated.