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Preparation and evaluation of a molecularly imprinted sol–gel material as the solid‐phase extraction adsorbents for the specific recognition of cloxacilloic acid in cloxacillin
Author(s) -
Du Kangli,
Luo Zhimin,
Guo Pengqi,
Tang Weili,
Wu Ningli,
Zheng Penglei,
Du Wei,
Zeng Aiguo,
Jing Wanghui,
Chang Chun,
Fu Qiang
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500961
Subject(s) - solid phase extraction , molecularly imprinted polymer , chromatography , cloxacillin , chemistry , adsorption , extraction (chemistry) , molecular imprinting , organic chemistry , selectivity , biochemistry , antibiotics , penicillin , catalysis
Highly selective molecularly imprinted polymers on the surface of silica gels were prepared by a sol–gel process and used as solid‐phase extraction adsorbents for the specific recognition, enrichment and detection of cloxacilloic acid in cloxacillin. The obtained polymers were characterized by scanning electron microscopy, FTIR spectroscopy, nitrogen adsorption and desorption, elemental analysis and thermogravimetric analysis. The imprinted polymers not only possessed high adsorption capacity (6.5 μg/mg), but also exhibited fast adsorption kinetics (they adsorb 80% of the maximum amount within 20 min) and excellent selectivity (the imprinted factor was 3.6). A method using the imprinted polymers as solid‐phase extraction adsorbents coupled with high‐performance liquid chromatography was established with good specificity, linearity ( r = 0.9962), precision (ranging from 0.5 to 6.7%), accuracy (ranging from 93.9 to 97.7%) and extraction recoveries (ranging from 78.8 to 89.8%). The limits of detection and quantification were 0.07 and 0.25 mg/g, respectively. This work could provide a promising method in the enrichment, extraction and detection of allergenic impurities in the manufacture, storage and application of cloxacillin.