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Simultaneous determination of aminopyrine and antipyrine in porcine muscle, milk, and eggs using liquid chromatography with tandem mass spectrometry
Author(s) -
Park JinA,
Zhang Dan,
Kim SeongKwan,
Cho SangHyun,
Cho SooMin,
Yi Hee,
Shim JaeHan,
Kim JinSuk,
Abd ElAty A. M.,
Shin HoChul
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500920
Subject(s) - chromatography , chemistry , analyte , detection limit , tandem mass spectrometry , mass spectrometry , residue (chemistry) , liquid chromatography–mass spectrometry , maximum residue limit , matrix (chemical analysis) , pesticide residue , pesticide , biochemistry , agronomy , biology
The concentrations of residual aminopyrine and antipyrine in porcine muscle, milk, and egg samples were analyzed using liquid chromatography with tandem mass spectrometry after undergoing a series of sample pretreatment steps. Owing to an ion suppression effect, matrix‐matched calibrations were used for analyte quantitation with determination coefficients ( R 2 ) ≥ 0.9931. The recovery rates for aminopyrine and antipyrine in various matrices at two spiking levels (5 and 10 ng/g) fell in the range of 60.96–68.87 and 61.87–66.99%, respectively. Meanwhile, the intra‐ and inter‐day precisions (expressed as relative standard deviation) were 1.02–12.95 and 1.71–5.50%, respectively. The method's detection limit (1 ng/g) was very low, thus enabling the detection of low residue levels. The applicability of the developed method was demonstrated with actual market samples and none of the tested analytes was detected in any of the samples.