Premium
Recovery and purification of chitosanase produced by Bacillus cereus using expanded bed adsorption and central composite design
Author(s) -
de Araújo Nathália Kelly,
Pimentel Vanessa Carvalho,
Silva Nayane Macedo Portela,
Araújo Padilha Carlos Eduardo,
de Macedo Gorete Ribeiro,
dos Santos Everaldo Silvino
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500900
Subject(s) - chitosanase , chromatography , adsorption , bacillus cereus , chemistry , yield (engineering) , central composite design , packed bed , cereus , response surface methodology , chitosan , materials science , bacteria , biology , biochemistry , composite material , organic chemistry , genetics
This study presents a system for expanded bed adsorption for the purification of chitosanase from broth extract in a single step. A chitosanase‐producing strain was isolated and identified as Bacillus cereus C‐01 and used to produce chitosanases. The expanded bed adsorption conditions for chitosanase purification were optimized statistically using STREAMLINE TM DEAE and a homemade column (2.6 × 30.0 cm). Dependent variables were defined by the quality criteria purification factor ( P ) and enzyme yield to optimize the chromatographic process. Statistical analyses showed that the optimum conditions for the maximum P were 150 cm/h load flow velocity, 6.0 cm settled bed height, and 7.36 cm distributor height. Distributor height had a strong influence on the process, considerably affecting both the P and enzyme yield. Optimizing the purification variables resulted in an approximately 3.66‐fold increase in the P compared with the value under nonoptimized conditions. This system is promising for the recovery of chitosanase from B. cereus C‐01 and is economically viable because it promotes the reduction steps.