Premium
Extraction of quinolones from milk samples using bentonite/magnetite nanoparticles before determination by high‐performance liquid chromatography with fluorimetric detection
Author(s) -
Jin Tao,
Wu Hao,
Gao Nannan,
Chen Xiaodan,
Lai Huajie,
Zheng Jinfeng,
Du Liming
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500856
Subject(s) - chromatography , detection limit , chemistry , danofloxacin , coprecipitation , solid phase extraction , extraction (chemistry) , enrofloxacin , calibration curve , flumequine , antibacterial agent , ciprofloxacin , inorganic chemistry , biochemistry , antibiotics
In this work, bentonite magnetic nanoparticles synthesized by a typical coprecipitation method were used as the adsorbent for the magnetic solid‐phase extraction of six quinolones (ciprofloxacin, difloxacin, enrofloxacin, norfloxacin, sarafloxacin, and lomefloxacin) from milk samples followed by high‐performance liquid chromatography with fluorimetric detection. Under the optimized conditions, the linear quantitation range for the six quinolones was 0.3–200 ng/mL, and the correlation coefficients of the calibration curves ranged from 0.9994 to 0.9999. The detection limit of the method was 0.1 ng/mL. Recoveries of quinolones from pure and low‐fat spiked milk samples varied from 80.4 to 92.7% and from 81.3 to 93.5%, respectively. These results demonstrated that the proposed method for the determination of six quinolones in milk samples was rapid, reliable, and efficient.