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Molecularly imprinted polymeric stir bar: Preparation and application for the determination of naftopidil in plasma and urine samples
Author(s) -
Peng Jun,
Xiao Deli,
He Hua,
Zhao Hongyan,
Wang Cuixia,
Shi Tian,
Shi Kexin
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500751
Subject(s) - molecularly imprinted polymer , chromatography , chemistry , elution , adsorption , sample preparation , polymer , detection limit , materials science , selectivity , organic chemistry , catalysis
In this study, molecularly imprinting technology and stir bar absorption technology were combined to develop a microextraction approach based on a molecularly imprinted polymeric stir bar. The molecularly imprinted polymer stir bar has a high performance, is specific, economical, and simple to prepare. The obtained naftopidil‐imprinted polymer‐coated bars could simultaneously agitate and adsorb naftopidil in the sample solution. The ratio of template/monomer/cross‐linker and conditions of template removal were optimized to prepare a stir bar with highly efficient adsorption. Fourier transform infrared spectroscopy, scanning electron microscopy, selectivity, and extraction capacity experiments showed that the molecularly imprinted polymer stir bar was prepared successfully. To utilize the molecularly imprinted polymer stir bar for the determination of naftopidil in complex body fluid matrices, the extraction time, stirring speed, eluent, and elution time were optimized. The limits of detection of naftopidil in plasma and urine sample were 7.5 and 4.0 ng/mL, respectively, and the recoveries were in the range of 90–112%. The within‐run precision and between‐run precision were acceptable (relative standard deviation <7%). These data demonstrated that the molecularly imprinted polymeric stir bar based microextraction with high‐performance liquid chromatography was a convenient, rapid, efficient, and specific method for the precise determination of trace naftopidil in clinical analysis.