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Approach based on high‐performance liquid chromatography fingerprint coupled with multivariate statistical analysis for the quality evaluation of Gastrodia Rhizoma
Author(s) -
Zhang Xuerong,
Ning Ziwan,
Ji De,
Chen Yi,
Mao Chunqin,
Lu Tulin
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500739
Subject(s) - gastrodia elata , chemistry , fumigation , chromatography , fingerprint (computing) , sulfur , active ingredient , principal component analysis , traditional medicine , traditional chinese medicine , mathematics , biology , organic chemistry , pharmacology , artificial intelligence , computer science , statistics , medicine , horticulture , alternative medicine , pathology
Gastrodia Rhizoma is a Traditional Chinese Medicine applied in the treatment of stroke, numbness of limb, headache and dizziness. However, its clinical effect is threatened by sulfur‐fumigation used in the process of storage. This article employs content determination coupled with high‐performance liquid chromatography fingerprint to investigate the effect of sulfur‐fumigation on Gastrodia Rhizoma so as to evaluate the quality of Gastrodia Rhizoma. The result was that most active ingredient in Gastrodia Rhizoma decreased after sulfur‐fumigation and the fingerprints analyzed by mathematical statistics between sulfur‐fumigated Gastrodia Rhizoma and unfumigated Gastrodia Rhizoma have substantial differences, which reveals that sulfur‐fumigation has a significant influence on the quality of Gastrodia Rhizoma. The conclusion of hierarchical clustering analysis, principal component analysis and partial least squares could validate each other, which implies that the method of mathematical statistics applied for assessing the quality of Gastrodia Rhizoma is effective and stable. The method not only affords a viable strategy for distinguishing Gastrodia Rhizoma whether sulfur‐fumigated or not and assessment of the quality of Gastrodia Rhizoma, but also provides a reference for other herbal medicine that suffers from sulfur‐fumigation.