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Rapid determination of flavonoids in licorice and comparison of three licorice species
Author(s) -
zhu Zhenhua,
Tao Weiwei,
Li Jianping,
Guo Sheng,
Qian Dawei,
Shang Erxin,
Su Shulan,
Duan Jinao
Publication year - 2016
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500685
Subject(s) - chromatography , chemistry , repeatability , detection limit , formic acid , high performance liquid chromatography , mass spectrometry , glycyrrhiza uralensis , calibration curve , gradient elution , analytical chemistry (journal) , medicine , alternative medicine , pathology
A simple, sensitive and fast method for the simultaneous quantitation of 15 flavonoids in licorice based on an ultra high performance liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry had been established and validated in this study. The analysis was performed on an ACQUITY HSS T3 column with gradient elution using a mobile phase consisted of A (0.1% formic acid in water)/B (acetonitrile) at a flow rate of 0.4 mL/min. Satisfactory separation of these compounds was obtained in less than 9 min. All calibration curves showed good linearity ( r 2 = 0.9940) during the test ranges. The precision, repeatability, accuracy, limit of detection and limit of quantification were also fully investigated. The validated method was successfully applied for the simultaneous quantitation of 15 flavonoids in 106 licorice samples which contained 83 batches of G. uralensis , 14 batches of G. glabra and 9 batches of G. inflata . Furthermore, multivariate statistical analysis (using principal components analysis) was performed to classify the samples based on the contents of the 15 analyzed compounds. The results showed that all of these licorice samples were rich in flavonoids, although their contents were obviously various, and the proposed method could serve as a prerequisite for quality control of licorice products.