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Interlaboratory study to evaluate the robustness of capillary electrophoresis‐mass spectrometry for peptide mapping
Author(s) -
Wenz Christian,
Barbas Coral,
LópezGonzálvez Ángeles,
Garcia Antonia,
Benavente Fernando,
SanzNebot Victoria,
Blanc Tim,
Freckleton Gordon,
BritzMcKibbin Philip,
Shanmuganathan Meera,
de l'Escaille Francois,
Far Johann,
Haselberg Rob,
Huang Sean,
Huhn Carolin,
Pattky Martin,
Michels David,
Mou Si,
Yang Feng,
Neusuess Christian,
Tromsdorf Nora,
Baidoo Edward E.K.,
Keasling Jay D.,
Park SungAe Suhr
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500551
Subject(s) - repeatability , chromatography , capillary electrophoresis , mass spectrometry , capillary electrophoresis–mass spectrometry , reproducibility , chemistry , capillary action , analytical chemistry (journal) , electrospray ionization , materials science , composite material
A collaborative study on the robustness and portability of a capillary electrophoresis‐mass spectrometry method for peptide mapping was performed by an international team, consisting of 13 independent laboratories from academia and industry. All participants used the same batch of samples, reagents and coated capillaries to run their assays, whereas they utilized the capillary electrophoresis‐mass spectrometry equipment available in their laboratories. The equipment used varied in model, type and instrument manufacturer. Furthermore, different types of sheath‐flow capillary electrophoresis–mass spectrometry interfaces were used. Migration time, peak height and peak area of ten representative target peptides of trypsin‐digested bovine serum albumin were determined by every laboratory on two consecutive days. The data were critically evaluated to identify outliers and final values for means, repeatability (precision within a laboratory) and reproducibility (precision between laboratories) were established. For relative migration time the repeatability was between 0.05 and 0.18% RSD and the reproducibility between 0.14 and 1.3% RSD. For relative peak area repeatability and reproducibility values obtained were 3–12 and 9–29% RSD, respectively. These results demonstrate that capillary electrophoresis‐mass spectrometry is robust enough to allow a method transfer across multiple laboratories and should promote a more widespread use of peptide mapping and other capillary electrophoresis‐mass spectrometry applications in biopharmaceutical analysis and related fields.