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Multi‐constituent determination and fingerprint analysis of Scutellaria indica L. using ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry
Author(s) -
Liang Xianrui,
Zhao Cui,
Su Weike
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500485
Subject(s) - quadrupole time of flight , fingerprint (computing) , mass spectrometry , chromatography , principal component analysis , chemistry , time of flight mass spectrometry , quality assessment , high performance liquid chromatography , retention time , analytical chemistry (journal) , tandem mass spectrometry , computer science , artificial intelligence , evaluation methods , ionization , ion , organic chemistry , engineering , reliability engineering
An ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry method integrating multi‐constituent determination and fingerprint analysis has been established for quality assessment and control of Scutellaria indica L. The optimized method possesses the advantages of speediness, efficiency, and allows multi‐constituents determination and fingerprint analysis in one chromatographic run within 11 min. 36 compounds were detected, and 23 of them were unequivocally identified or tentatively assigned. The established fingerprint method was applied to the analysis of ten S . indica samples from different geographic locations. The quality assessment was achieved by using principal component analysis. The proposed method is useful and reliable for the characterization of multi‐constituents in a complex chemical system and the overall quality assessment of S . indica .