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Development of a fabric phase sorptive extraction with high‐performance liquid chromatography and ultraviolet detection method for the analysis of alkyl phenols in environmental samples
Author(s) -
Kumar Rajesh,
Kabir Abuzar,
Furton Kenneth G.,
Malik Ashok Kumar
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500464
Subject(s) - chromatography , extraction (chemistry) , chemistry , detection limit , alkylphenol , solid phase extraction , high performance liquid chromatography , alkyl , phenols , matrix (chemical analysis) , organic chemistry
A novel analytical method has been developed and validated for the quantification of alkyl phenols in aqueous and soil samples. Fabric phase sorptive extraction, a new sorptive microextraction technique, has been employed for the preconcentration of some endocrine‐disruptor alkylphenol molecules, namely, 4‐ tert ‐butylphenol, 4‐ sec ‐butylphenol, 4‐ tert ‐amylphenol, and 4‐cumylphenol, followed by high‐performance liquid chromatography with ultraviolet detection. Various parameters influencing the fabric phase sorptive extraction performance, namely, extraction time, eluting solvent, elution time and pH of the sample matrix, were optimized. The chromatographic separation was carried out with a mobile phase of acetonitrile/water (60:40 v/v) at an isocratic flow rate of 1.0 mL/min using a reversed‐phase C 18 column at λ max 225 nm. The calibration curves of target analytes were prepared in the concentration range 5–500 ng/mL with good coefficient of determination values ( R 2 > 0.992). Extraction efficiency values were 74.0, 75.6, 78.0, and 78.3 for 4‐ tert ‐butylphenol, 4‐ sec ‐butylphenol, 4‐ tert ‐amylphenol, and 4‐cumylphenol, respectively. The limits of detection range from 0.161 to 0.192 ng/mL. Subsequently, the new fabric phase sorptive extraction with high‐performance liquid chromatography and ultraviolet detection was successfully applied for the recovery of alkyl phenols from spiked ground water, river water, and treated water from a sewage treatment plant, and soil and sludge samples.