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Fingerprint analysis, multi‐component quantitation, and antioxidant activity for the quality evaluation of Salvia miltiorrhiza var . alba by high‐performance liquid chromatography and chemometrics
Author(s) -
Zhang Danlu,
Duan Xiaoju,
Deng Shuhong,
Nie Lei,
Zang Hengchang
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500451
Subject(s) - salvia miltiorrhiza , rosmarinic acid , chemometrics , repeatability , chromatography , principal component analysis , chemistry , high performance liquid chromatography , fingerprint (computing) , antioxidant , artificial intelligence , computer science , traditional chinese medicine , biochemistry , medicine , alternative medicine , pathology
Salvia miltiorrhiza Bge. var. alba C.Y. Wu and H.W. Li has wide prospects in clinical practice. A useful comprehensive method was developed for the quality evaluation of S. miltiorrhiza var. alba by three quantitative parameters: high‐performance liquid chromatography fingerprint, ten‐component contents, and antioxidant activity. The established method was validated for linearity, precision, repeatability, stability, and recovery. Principal components analysis and hierarchical clustering analysis were both used to evaluate the quality of the samples from different origins. The results showed that there were category discrepancies in quality of S. miltiorrhiza var. alba samples according to the three quantitative parameters. Multivariate linear regression was adopted to explore the relationship between components and antioxidant activity. Three constituents, namely, danshensu, rosmarinic acid, and salvianolic acid B, significantly correlated with antioxidant activity, and were successfully elucidated by the optimized multivariate linear regression model. The combined use of high‐performance liquid chromatography fingerprint analysis, simultaneous multicomponent quantitative analysis, and antioxidant activity for the quality evaluation of S. miltiorrhiza var. alba is a reliable, comprehensive, and promising approach, which might provide a valuable reference for other herbal products in general to improve their quality control.