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Preparation and characterization of macroporous monoliths imprinted with erythromycin
Author(s) -
Vlakh E.G.,
Stepanova M.A.,
Pisarev O.A.,
Tennikova T.B.
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500438
Subject(s) - chromatography , ethylene glycol dimethacrylate , copolymer , erythromycin , materials science , methacrylate , molecular imprinting , porosimetry , scanning electron microscope , chemical engineering , chemistry , porosity , porous medium , methacrylic acid , polymer , selectivity , organic chemistry , composite material , biochemistry , catalysis , antibiotics , engineering
The synthesis of macroporous molecularly imprinted monoliths was performed using the monomers system 2‐hydroxyethyl methacrylate‐ethylene glycol dimethacrylate and erythromycin as a template. The copolymerization was carried out in situ inside 50 mm × 4.6 mm i.d. stainless‐steel tubing. The morphology of the monoliths was examined with scanning electron microscopy. The porous characteristics were determined both from the data of hydrodynamic permeability of monoliths and by means of mercury intrusion porosimetry. The retention parameters of target substance (erythromycin), values of calculated imprinting factors and apparent dynamic dissociation constants were obtained for monoliths prepared with the application of different amount of template (4, 8 and 12 mol%). The separations of the mixtures azithromycin/erythromycin and ciprofloxacin/erythromycin were demonstrated. Additionally, the possibility of erythromycin quantification in human blood plasma was shown.