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Capillary electrophoretic methods applied to the investigation of peptide complexes
Author(s) -
Štěpánová Sille,
Kašička Václav
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500399
Subject(s) - capillary electrophoresis , affinity electrophoresis , electrophoresis , chemistry , chromatography , capillary action , peptide , free flow electrophoresis , analytical chemistry (journal) , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , affinity chromatography , biochemistry , materials science , composite material , enzyme
This article gives an overview of the applications of capillary electrophoretic methods to investigate the non‐covalent interactions of peptides (peptide complexes) with variable middle‐ and high‐molecular‐mass receptors (ligands) as well as with small ions and molecules in the period 2007–2014. Different modes of capillary electrophoretic methods, such as mobility shift (vacancy) affinity capillary electrophoresis, multiple injection affinity capillary electrophoresis, partial filling affinity capillary electrophoresis, Hummel–Dryer method, vacancy peak method and (continuous) frontal analysis capillary electrophoresis, are briefly described and their applicability to determination of binding constants of peptide complexes is discussed. In addition, the detailed experimental conditions of individual applications and the values of binding constants of the particular peptide complexes are presented.