Premium
Quality‐by‐design‐based ultra high performance liquid chromatography related substances method development by establishing the proficient design space for sumatriptan and naproxen combination
Author(s) -
Patel Prinesh N,
Karakam Vijaya Saradhi,
Samanthula Gananadhamu,
Ragampeta Srinivas
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500343
Subject(s) - quality by design , chromatography , fractional factorial design , ammonium acetate , factorial experiment , design of experiments , acetonitrile , high performance liquid chromatography , chemometrics , elution , chemistry , analyte , volumetric flow rate , critical quality attributes , central composite design , analytical chemistry (journal) , mathematics , response surface methodology , statistics , quantum mechanics , particle size , physics
Quality‐by‐design‐based methods hold greater level of confidence for variations and greater success in method transfer. A quality‐by‐design‐based ultra high performance liquid chromatography method was developed for the simultaneous assay of sumatriptan and naproxen along with their related substances. The first screening was performed by fractional factorial design comprising 44 experiments for reversed‐phase stationary phases, pH, and organic modifiers. The results of screening design experiments suggested phenyl hexyl column and acetonitrile were the best combination. The method was further optimized for flow rate, temperature, and gradient time by experimental design of 20 experiments and the knowledge space was generated for effect of variable on response (number of peaks ≥ 1.50 – resolution). Proficient design space was generated from knowledge space by applying Monte Carlo simulation to successfully integrate quantitative robustness metrics during optimization stage itself. The final method provided the robust performance which was verified and validated. Final conditions comprised Waters® Acquity phenyl hexyl column with gradient elution using ammonium acetate (pH 4.12, 0.02 M) buffer and acetonitrile at 0.355 mL/min flow rate and 30°C. The developed method separates all 13 analytes within a 15 min run time with fewer experiments compared to the traditional quality‐by‐testing approach.