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The quest for affinity chromatography ligands: are the molecular libraries the right source?
Author(s) -
Perret Gérald,
Santambien Patrick,
Boschetti Egisto
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500285
Subject(s) - affinity chromatography , chemistry , ligand (biochemistry) , identification (biology) , combinatorial chemistry , computational biology , affinity label , chromatography , binding site , biochemistry , receptor , biology , botany , enzyme
Affinity chromatography separations of proteins call for highly specific ligands. Antibodies are the most obvious approach; however, except for specific situations, technical and economic reasons are arguments against this choice especially for preparative purposes. With this in mind, the rationale is to select the most appropriate ligands from collections of pre‐established molecules. To reach the objective of having a large structural coverage, combinatorial libraries have been proposed. These are classified according to their nature and origin. This review presents and discusses the most common affinity ligand libraries along with the most appropriate screening methods for the identification of the right affinity chromatography selective structure according to the type of library; a side‐by‐side comparison is also presented.