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Determination of furanic aldehydes in sugarcane bagasse by high‐performance liquid chromatography with pulsed amperometric detection using a modified electrode with nickel nanoparticles
Author(s) -
da Silva José Luiz,
Beluomini Maísa Azevedo,
Stradiotto Nelson Ramos
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500253
Subject(s) - amperometry , furfural , chromatography , detection limit , chemistry , bagasse , hydroxymethylfurfural , high performance liquid chromatography , repeatability , nickel , analytical chemistry (journal) , electrode , electrochemistry , organic chemistry , ecology , biology , catalysis
A glassy carbon electrode chemically modified with nickel nanoparticles coupled with reversed‐phase chromatography with pulsed amperometric detection was used for the quantitative analysis of furanic aldehydes in a real sample of sugarcane bagasse hydrolysate. Chromatographic separation was carried out in isocratic conditions (acetonitrile/water, 1:9) with a flow rate of 1.0 mL/min, a detection potential of – 50 mV vs. Pd, and the process was completed within 4 min. The analytical curves presented limits of detection of 4.0 × 10 −7 mol/L and 4.3 × 10 −7 mol/L, limits of quantification of 1.3 × 10 −6 and 1.4 × 10 −6 mol/L, amperometric sensitivities of 2.2 × 10 6 nA mol/L and 2.7 × 10 6 nA mol/L for furfural and 5‐hydroxymethylfurfural, respectively. The values obtained in this sample by the standard addition method were 1.54 ± 0.02 g/kg for 5‐hydroxymethylfurfural and 11.5 ± 0.2 g/kg for furfural. The results demonstrate that this new proposed method can be used for the quick detection of furanic aldehydes without the interference of other electroactive species, besides having other remarkable merits that include excellent peak resolution, analytical repeatability, sensitivity, and accuracy.