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Isolation and purification of prenylated phenolics from Amorpha fruticosa by high‐speed counter‐current chromatography
Author(s) -
Chen Chu,
Wu Yan,
Chen Yang,
Du Leilei
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500224
Subject(s) - chemistry , chromatography , countercurrent chromatography , prenylation , mass spectrometry , high performance liquid chromatography , hexane , solvent , ethanol , nuclear magnetic resonance spectroscopy , stereochemistry , organic chemistry , enzyme
Prenylated phenolics such as amorfrutins are recently identified potent anti‐inflammatory and antidiabetic natural products. In this work, high‐speed counter‐current chromatography was investigated for the isolation and purification of prenylated phenolics from the fruits of Amorpha fruticosa by using a two‐phase solvent system composed of n ‐hexane/ethanol/water (5:4:1, v/v). As a result, 14.2 mg of 5,7‐dihydroxy‐8‐geranylflavanone, 10.7 mg of amorfrutin A and 17.4 mg of amorfrutin B were obtained from 200 mg of n ‐hexane‐soluble crude extract in one step within 250 min. The purities of 5,7‐dihydroxy‐8‐geranylflavanone, amorfrutins A and B were 95.2, 96.7 and 97.1%, respectively, as determined by ultra high performance liquid chromatography. The structural identification was performed by mass spectrometry and 1 H and 13 C NMR spectroscopy. The results indicated that the established method is an efficient and convenient way to purified prenylated phenolics from A. fruticosa extract.