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Evaluation of two molecularly imprinted polymers for the solid‐phase extraction of natural, synthetic and mycoestrogens from environmental water samples before liquid chromatography with mass spectrometry
Author(s) -
GonzálezSálamo Javier,
SocasRodríguez Bárbara,
HernándezBorges Javier,
Afonso María,
RodríguezDelgado Miguel Ángel
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500194
Subject(s) - chromatography , solid phase extraction , chemistry , molecularly imprinted polymer , extraction (chemistry) , mass spectrometry , electrospray , analyte , electrospray ionization , detection limit , sample preparation , zearalenone , selectivity , organic chemistry , food science , aflatoxin , catalysis
In this work, we have compared the selectivity of two commercial molecularly imprinted polymers (AFFINIMIP®SPE Estrogens and AFFINIMIP®SPE Zearalenone) for the extraction of 12 estrogenic compounds of interest (i.e. 17α‐estradiol, 17β‐estradiol, estrone, hexestrol, 17α‐ethynylestradiol, diethylstibestrol, dienestrol, zearalenone, α‐zearalanol, β‐zearalanol, α‐zearalenol and β‐zearalenol) from different water samples. High‐performance liquid chromatography coupled with ion trap mass spectrometry with electrospray ionization was used for their determination. Results showed that although both molecularly imprinted polymeric cartridges were specifically designed for different groups of analytes (natural estrogens like estradiol in the first case and zearalenone derivatives in the second) they nearly have the same extraction performance (with recovery values in the range 65–101%) for the same analytes in Milli‐Q water because of the cross‐reactivity of the polymer. However, when more complex water samples were analyzed, it was clear that the behavior was different and that the AFFINIMIP®SPE Estrogens showed less cross‐reactivity than the other cartridge. Validation of the proposed methodology with both cartridges revealed that the extraction was reproducible and that the final limits of detection of the proposed method were in the low ng/L range.