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Porous silica particles grafted with an amphiphilic side‐chain polymer as a stationary phase in reversed‐phase high‐performance liquid chromatography
Author(s) -
Shahruzzaman Md.,
Takafuji Makoto,
Ihara Hirotaka
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500189
Subject(s) - amphiphile , polymer , chemistry , phase (matter) , side chain , selectivity , fourier transform infrared spectroscopy , hydrophilic interaction chromatography , reversed phase chromatography , silica gel , high performance liquid chromatography , chemical engineering , polymer chemistry , organic chemistry , chromatography , copolymer , catalysis , engineering
The amphiphilic polymer‐grafted silica was newly prepared as a stationary phase in high‐performance liquid chromatography. Poly(4‐vinylpyridine) with a trimethoxysilyl group at one end was grafted onto porous silica particles and the pyridyl side chains were quaternized with 1‐bromooctadecane. The obtained poly(octadecylpyridinium)‐grafted silica was characterized by elemental analysis, diffuse reflectance infrared Fourier transform spectroscopy and Brunauer–Emmett–Teller analysis. The degree of quaternization of the pyridyl groups on the obtained stationary phase was estimated to be 70%. The selective retention behaviors of polycyclic aromatic hydrocarbons including some positional isomers were investigated using poly(octadecylpyridinium)‐grafted silica as an amphiphilic polymer stationary phase in high‐performance liquid chromatography and results were compared with commercially available polymeric octadecylated silica and phenyl‐bonded silica columns. The results indicate that the selectivity toward polycyclic aromatic hydrocarbons exhibited by the amphiphilic polymer stationary phase is higher than the corresponding selectivity exhibited by a conventional phenyl‐bonded silica column. However, compared with the polymeric octadecylated silica phase, the new stationary phase presents similar retention behavior for polycyclic aromatic hydrocarbons but different retention behavior particularly for positional isomers of disubstituted benzenes as the aggregation structure of amphiphilic polymers on the surface of silica substrate has been altered during mobile phase variation.