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Comprehensive analysis of fatty alcohol ethoxylates by ultra high pressure hydrophilic interaction chromatography coupled with ion mobility spectrometry mass spectrometry using a custom‐designed sub‐2 μm column
Author(s) -
Ma Qiang,
Ma Wei,
Chen Xi,
Wang Ziming,
Bai Hua,
Zhang Lanwei,
Li Wentao,
Wang Chao,
Li Xinshi
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500185
Subject(s) - chemistry , ion mobility spectrometry , chromatography , mass spectrometry , fatty alcohol , resolution (logic) , alcohol , gas chromatography , analytical chemistry (journal) , organic chemistry , artificial intelligence , computer science
Comprehensive analysis of fatty alcohol ethoxylates has been conducted by coupling ultra high pressure hydrophilic interaction chromatography and ion mobility spectrometry mass spectrometry. A custom‐designed sub‐2 μm column was used for the chromatographic separation of fatty alcohol ethoxylates by hydrophilic interaction chromatography. Ion mobility spectrometry provided a post‐ionization resolution during a very short period of 6.4 ms. Distinguishable families of singly, doubly, and triply charged fatty alcohol ethoxylates were clearly observed. By virtue of the combination of hydrophilic interaction chromatography and ion mobility spectrometry, comprehensive resolution based on both hydrophobicity difference and mobility disparity has been achieved for fatty alcohol ethoxylates. The orthogonality of the developed separation and analysis system was evaluated with the correlation coefficient and peak spreading angle of 0.0224 and 88.72°, respectively. The actual peak capacity obtained was individually 40 and 193 times than those when hydrophilic interaction chromatography and ion mobility spectrometry were used alone. The collision cross‐sections of fatty alcohol ethoxylates were calculated by calibrating the traveling wave ion mobility device with polyalanine.