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Determination of zearalenone in maize products by vortex‐assisted ionic‐liquid‐based dispersive liquid–liquid microextraction with high‐performance liquid chromatography
Author(s) -
Wang Lin,
Luan Chuanlei,
Chen Fangfang,
Wang Rui,
Shao Lihua
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500184
Subject(s) - chromatography , zearalenone , ionic liquid , chemistry , liquid liquid , high performance liquid chromatography , mycotoxin , organic chemistry , food science , catalysis
A novel method has been developed for the analysis of zearalenone in maize products by vortex‐assisted ionic‐liquid‐based dispersive liquid–liquid microextraction combined with HPLC and fluorescence detection. Maize samples were extracted with methanol/water (80:20, v/v) and the extraction solution was then used as the dispersive solvent in the microextraction procedure. The analyte was rapidly transmitted to a small volume of ionic liquid and was determined by HPLC. Various parameters affecting the recovery of the mycotoxin were investigated, such as the type and volume of the extraction solvent, the type and volume of the dispersive solvent, the pH of the aqueous phase, the salt addition, and the time of vortex and centrifugation. Under the optimal experimental conditions, a good linearity of the analyte was obtained in the range of 1.0–1000.0 μg/L with the correlation coefficient of 0.9998. The limit of detection (S/N = 3) and quantification (S/N = 10) were 0.3 and 1.0 μg/kg, and the mean recoveries ranged from 83.5 to 94.9%, with a relative standard deviation less than 5.0%. The proposed method was demonstrated to be simple, cheap, quick, and highly selective and was successfully applied to the determination of zearalenone in maize products.