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Development and validation of a quantification method for ziyuglycoside I and II in rat plasma: Application to their pharmacokinetic studies
Author(s) -
Ye Wei,
Fu Hanxu,
Xie Lin,
Zhou Lijun,
Rao Tai,
Wang Qian,
Shao Yuhao,
Xiao Jingcheng,
Kang Dian,
Wang Guangji,
Liang Yan
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500102
Subject(s) - chromatography , chemistry , analyte , pharmacokinetics , mass spectrometry , extraction (chemistry) , tandem mass spectrometry , liquid chromatography–mass spectrometry , matrix (chemical analysis) , elution , high performance liquid chromatography , detection limit , medicine
This study provided a novel and generally applicable method to determine ziyuglycoside I and ziyuglycoside II in rat plasma based on liquid chromatography with tandem mass spectrometry. A single step of liquid–liquid extraction with n ‐butanol was utilized, and ginsenoside Rg3 was chosen as internal standard. Final extracts were analyzed based on liquid chromatography with tandem mass spectrometry. Chromatographic separation was achieved using a Thermo Golden C 18 column, and the applied gradient elution program allowed for the simultaneous determination of two ziyuglycosides in a one‐step chromatographic separation with a total run time of 10 min. The fully validated methodology for both analytes demonstrated high sensitivity (the lower limit of quantitation was 2.0 ng/mL), good accuracy (% RE ≤ ± 15) and precision (% RSD ≤ 15). The average recoveries of both ziyuglycosides and internal standard were all above 75% and no obvious matrix effect was found. This method was then successfully applied to the preclinical pharmacokinetic studies of ziyuglycoside I and ziyuglycoside II. The presently developed methodology would be useful for the preclinical and clinical pharmacokinetic studies for ziyuglycoside I and ziyuglycoside II.