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Separation and purification of five alkaloids from Aconitum duclouxii by counter‐current chromatography
Author(s) -
Wang Yarong,
Cai Shining,
Chen Yang,
Deng Liang,
Zhou Xumei,
Liu Jia,
Xu Xin,
Xia Qiang,
Lin Mao,
Zhang Jili,
Huang Weili,
Wang Wenjun,
Xiang Canhui,
Cui Guozhen,
Du Lianfeng,
He Huan,
Qi Baohui
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500059
Subject(s) - aconitum , chromatography , chemistry , countercurrent chromatography , aconitine , extraction (chemistry) , ethyl acetate , methanol , hexane , solvent , high performance liquid chromatography , alkaloid , organic chemistry
C 19 ‐diterpenoid alkaloids are the main components of Aconitum duclouxii Levl. The process of separation and purification of these compounds in previous studies was tedious and time consuming, requiring multiple chromatographic steps, thus resulted in low recovery and high cost. In the present work, five C 19 ‐diterpenoid alkaloids, namely, benzoylaconine ( 1 ), N‐ deethylaconitine ( 2 ), aconitine ( 3 ), deoxyaconitine ( 4 ), and ducloudine A ( 5 ), were efficiently prepared from A. duclouxii Levl ( Aconitum L.) by ethyl acetate extraction followed with counter‐current chromatography. In the process of separation, the critical conditions of counter‐current chromatography were optimized. The two‐phase solvent system composed of n ‐hexane/ethyl acetate/methanol/water/NH 3 ·H 2 O (25%) (1:1:1:1:0.1, v/v) was selected and 148.2 mg of 1 , 24.1 mg of 2 , 250.6 mg of 3 , 73.9 mg of 4, and 31.4 mg of 5 were obtained from 1 g total Aconitum alkaloids extract, respectively, in a single run within 4 h. Their purities were found to be 98.4, 97.2, 98.2, 96.8, and 96.6%, respectively, by ultra‐high performance liquid chromatography analysis. The presented separation and purification method was simple, fast, and efficient, and the obtained highly pure alkaloids are suitable for biochemical and toxicological investigation.