Premium
Rapidly differentiating grape seeds from different sources based on characteristic fingerprints using direct analysis in real time coupled with time‐of‐flight mass spectrometry combined with chemometrics
Author(s) -
Song Yuqiao,
Liao Jie,
Dong Junxing,
Chen Li
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201500055
Subject(s) - chemometrics , principal component analysis , mass spectrometry , hierarchical clustering , grape seed , chemistry , dart ion source , grape wine , chromatography , wine , cluster analysis , analytical chemistry (journal) , biological system , mathematics , food science , statistics , ion , biology , organic chemistry , electron ionization , ionization
The seeds of grapevine ( Vitis vinifera ) are a byproduct of wine production. To examine the potential value of grape seeds, grape seeds from seven sources were subjected to fingerprinting using direct analysis in real time coupled with time‐of‐flight mass spectrometry combined with chemometrics. Firstly, we listed all reported components (56 components) from grape seeds and calculated the precise m / z values of the deprotonated ions [M–H] – . Secondly, the experimental conditions were systematically optimized based on the peak areas of total ion chromatograms of the samples. Thirdly, the seven grape seed samples were examined using the optimized method. Information about 20 grape seed components was utilized to represent characteristic fingerprints. Finally, hierarchical clustering analysis and principal component analysis were performed to analyze the data. Grape seeds from seven different sources were classified into two clusters; hierarchical clustering analysis and principal component analysis yielded similar results. The results of this study lay the foundation for appropriate utilization and exploitation of grape seed samples. Due to the absence of complicated sample preparation methods and chromatographic separation, the method developed in this study represents one of the simplest and least time‐consuming methods for grape seed fingerprinting.