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Comparative fingerprint analyses of extracts from the root bark of wild Hippocratea excelsa and “Cancerina” by high‐performance liquid chromatography
Author(s) -
Araujo Leon JesusAlfredo,
Ruiz Ciau DurcyVerenice,
Coral Martinez TaniaIsolina,
Cantillo Ciau ZulemaOsiris
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201401480
Subject(s) - bark (sound) , chromatography , chemistry , celastraceae , high performance liquid chromatography , juglandaceae , fingerprint (computing) , gradient elution , resolution (logic) , juglans , biology , stereochemistry , computer science , ecology , biochemistry , computer security , artificial intelligence
A simple high‐performance liquid chromatography method was developed for the identification and comparison of quinone‐methide triterpenes in wild Hippocratea excelsa and “cancerina” to establish the chromatographic profile of these compounds in root bark. The essential chromatographic conditions for this method are based on a gradient system with a reversed‐phase column (C 18 ) using proportions of water, methanol, and tetrahydrofuran as mobile phases to correctly separate the signals at 254 and 420 nm and compare the signals to those reported in the literature. The chromatograms exhibit good resolution and precision. Statistical analysis showed that the chromatographic profiles of wild H. excelsa and cancerina do not exhibit significant differences ( p≥0.05) in their area proportions or relative retention times. The method developed in this study is suitable for the identification of the major chemotaxonomic markers of the Celastraceae family and can be used for quality control of this herbal root bark, which has uses today in Mexican folk medicine.