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Evaluation of a silicon oxynitride hydrophilic interaction liquid chromatography column in saccharide and glycoside separations
Author(s) -
Wan Huihui,
Sheng Qianying,
Zhong Hongmin,
Guo Xiujie,
Fu Qing,
Liu Yanfang,
Xue Xingya,
Liang Xinmiao
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201401413
Subject(s) - chemistry , silicon oxynitride , hydrophilic interaction chromatography , chromatography , high performance liquid chromatography , reversed phase chromatography , phase (matter) , silicon , selectivity , chromatography column , organic chemistry , silicon nitride , catalysis
The retention characteristics of a silicon oxynitride stationary phase for carbohydrate separation were studied in hydrophilic interaction chromatography mode. Four saccharides including mono‐, di‐, and trisaccharides were employed to investigate the effects of water content and buffer concentration in the mobile phase on hydrophilic interaction liquid chromatography retention. For the tested saccharides, the silicon oxynitride column demonstrated excellent performance in terms of separation efficiency, hydrophilicity, and interesting separation selectivity for carbohydrates compared to the bare silica stationary phase. Finally, the silicon oxynitride hydrophilic interaction liquid chromatography column was employed in the separation of complex samples of fructooligosaccharides, saponins, and steviol glycoside from natural products. The resulting chromatograms demonstrated good separation efficiency and longer retention compared with silica, which further confirmed the advantages and potential application of silicon oxynitride stationary phase for hydrophilic interaction liquid chromatography separation.