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Selenium speciation in tea by dispersive liquid–liquid microextraction coupled to high‐performance liquid chromatography after derivatization with 2,3‐diaminonaphthalene
Author(s) -
Zhou Qingxiang,
Lei Man,
Li Jing,
Wang Mengyun,
Zhao Danchen,
Xing An,
Zhao Kuifu
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201401373
Subject(s) - chromatography , derivatization , selenium , chemistry , detection limit , high performance liquid chromatography , chlorobenzene , acetonitrile , solvent , extraction (chemistry) , reagent , disperser , materials science , organic chemistry , catalysis , composite material
Selenium is an important element for human health, and it is present in many natural drinks and foods. Present study described a new method using dispersive liquid–liquid microextraction prior to high‐performance liquid chromatography with a UV variable wavelength detector for the determination of the total selenium, Se(IV), Se(VI), and total organoselenium in tea samples. In the procedure, 2,3‐diaminonaphthalene was used as the chelating reagent, 400 μL acetonitrile was used as the disperser solvent and 60 μL chlorobenzene was used as the extraction solvent. The complex of Se(IV) and 2,3‐diaminonaphthalene in the final extracted phase was analyzed by high‐performance liquid chromatography. The factors influencing the derivatization and microextraction were investigated. Under the optimal conditions, the limit of detection was 0.11 μg/L for Se(IV) and the linearity range was in the range of 0.5–40 μg/L. This method was successfully applied to the determination of selenium in four tea samples with spiked recoveries ranging from 91.3 to 100%.