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Determination of the triterpene glycosides in sea cucumbers by liquid chromatography with evaporative light scattering and mass spectrometry detection
Author(s) -
Yang Jie,
Wang Yuanhong,
Zhang Ran,
Jiang Tifngfu,
Lv Zhihua
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201401253
Subject(s) - triterpene , chemistry , glycoside , chromatography , mass spectrometry , electrospray ionization , sea cucumber , extraction (chemistry) , liquid chromatography–mass spectrometry , organic chemistry , biology , medicine , ecology , alternative medicine , pathology
Holothurian triterpene glycosides possess various kinds of biological activities, including antifungal, cytotoxic, hemolytic, cytostatic, and immunomodulatory effects. In this study, a rapid extraction method of triterpene glycosides from sea cucumbers using a small column of C 18 solid phase was first developed. Furthermore, a novel high‐performance liquid chromatography method coupled with evaporative light scattering detection and electrospray ionization mass spectrometry was established for the determination of each triterpene glycosides from different sea cucumbers. Simultaneous separation of all kind of triterpene glycoside were achieved on a C 18 column. A gradient of aqueous acetonitrile was applied, and the method was validated. The liquid chromatography method was applied to the online mass detection to identify the triterpene glycosides in the purified extraction of eight kinds of pulverized sea cucumber from the market of Qingdao, China. The negative mode of [M–H] − /[M–Na] − exclusively shown signals corresponding to the triterpene glycosides previously reported and the MS 2 product ions of those ions indicate the specific structure of each triterpene glycoside.