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Evaluation of chromatographic columns packed with semi‐ and fully porous particles for benzimidazoles separation
Author(s) -
GonzaloLumbreras Raquel,
SanzLandaluze Jon,
Cámara Carmen
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201401054
Subject(s) - chromatography , resolution (logic) , fractional factorial design , chemistry , factorial experiment , particle size , column (typography) , selectivity , monolithic hplc column , chromatographic separation , benzimidazole , porosity , analytical chemistry (journal) , materials science , high performance liquid chromatography , mathematics , computer science , organic chemistry , statistics , geometry , connection (principal bundle) , artificial intelligence , catalysis
The behavior of 15 benzimidazoles, including their main metabolites, using several C 18 columns with standard or narrow‐bore diameters and different particle size and type were evaluated. These commercial columns were selected because their differences could affect separation of benzimidazoles, and so they can be used as alternative columns. A simple screening method for the analysis of benzimidazole residues and their main metabolites was developed. First, the separation of benzimidazoles was optimized using a Kinetex C 18 column; later, analytical performances of other columns using the above optimized conditions were compared and then individually re‐optimized. Critical pairs resolution, analysis run time, column type and characteristics, and selectivity were considered for chromatographic columns comparison. Kinetex XB was selected because it provides the shortest analysis time and the best resolution of critical pairs. Using this column, the separation conditions were re‐optimized using a factorial design. Separations obtained with the different columns tested can be applied to the analysis of specific benzimidazoles residues or other applications.