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Screening of drugs of abuse and toxic compounds in human whole blood using online solid‐phase extraction and high‐performance liquid chromatography with time‐of‐flight mass spectrometry
Author(s) -
Teng Xiaomei,
Liang Chen,
Wang Rong,
Sun Tao,
Rao Yulan,
Ni Chunfang,
Zeng Libo,
Xiong Lingjuan,
Li Yuan,
Zhang Yurong
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400963
Subject(s) - chromatography , mass spectrometry , solid phase extraction , detection limit , chemistry , analyte , time of flight mass spectrometry , extraction (chemistry) , drugs of abuse , time of flight , selected ion monitoring , ion , gas chromatography–mass spectrometry , drug , pharmacology , medicine , organic chemistry , ionization
A novel method for the screening of 151 drugs of abuse and toxic compounds in human whole blood has been developed and validated by online solid‐phase extraction with liquid chromatography coupled to time‐of‐flight mass spectrometry. Analytes were extracted and separated by using a fully automated online solid‐phase extraction liquid chromatography system with total chromatographic run time of 26 min. Time‐of‐flight mass spectrometry screening of 151 drugs of abuse and toxic compounds was performed in a full‐scan ( m/z 50–800) mode using an MS E acquisition of molecular ions and fragment ions data at two collision energies (one was 6 eV and another one was in the range of 5–45 eV). The compounds were identified based on retention times and exact mass of molecular ions and fragment ions. The limit of detection ranged from 1 to 100 ng/mL and the recovery of the method ranged from 6.3 to 163.5%. This method is proved to be a valuable screening method allowing fast and specific identification of drugs in human whole blood.