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Development and validation of a liquid chromatography with mass spectrometry method to determine resveratrol and piceid isomers in beeswax
Author(s) -
Ares Ana M.,
González Yolanda,
Nozal María J.,
Bernal José L.,
Higes Mariano,
Bernal José
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400955
Subject(s) - chromatography , chemistry , mass spectrometry , extraction (chemistry) , ammonium formate , beeswax , electrospray , analyte , detection limit , electrospray ionization , solid phase extraction , calibration curve , analytical chemistry (journal) , wax , organic chemistry
This paper represents the first report of a liquid chromatography coupled to electrospray ionization mass spectrometry method for simultaneously analyzing resveratrol and piceid isomers ( cis and trans ) in beeswax. An efficient extraction procedure has been proposed (average analyte recoveries were between 89 and 95%); this involved a solid–liquid extraction using a mixture of ethanol and water (80:20, v/v) and a concentration step in a rotary evaporator. The separation of all the compounds was achieved using a C 18 column and a mobile phase composed of ammonium formate 0.03 M in water and acetonitrile in gradient elution mode at a flow rate of 1 mL/min. The method was fully validated in terms of selectivity, limits of detection and quantification, linearity, precision, and accuracy. The limits of detection and quantification ranged from 1.0 to 1.7 and 3.5 to 5.5 μg/kg, respectively. Finally, the proposed method was applied to analyze beeswax samples collected from experimental and organic apiaries.