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Hydrophilic solid‐phase extraction of melamine with ampholine‐modified hybrid organic–inorganic silica material
Author(s) -
Wang Tingting,
Zhu Yiming,
Ma Junfeng,
Xuan Rongrong,
Gao Haoqi,
Liang Zhen,
Zhang Lihua,
Zhang Yukui
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400900
Subject(s) - melamine , sorbent , elution , solid phase extraction , extraction (chemistry) , detection limit , chromatography , solvent , chemistry , adsorption , materials science , organic chemistry
In this work, an ampholine‐functionalized hybrid organic–inorganic silica sorbent was successfully used to extract melamine from a milk formula sample by a hydrophilic interaction solid‐phase extraction protocol. Primary factors affecting the extraction efficiency of the material such as extraction solvent, elution solvent, sample loading volume, and elution volume have been thoroughly optimized. Under the optimized hydrophilic solid‐phase extraction conditions, the recoveries of melamine spiked in milk formula samples ranged from 86.2 to 101.8% with relative standard deviations of 4.1–9.4% ( n = 3). The limit of detection (S/N = 3) was 0.32 μg/g. The adsorption capacity toward melamine was 30 μg of melamine per grams of sorbent. Due to its simplicity, rapidity and cost effectiveness, the newly developed hydrophilic solid‐phase extraction method should provide a promising tool for daily monitoring of doped melamine in milk formula.