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Development of a decaaza‐cyclophane stationary phase for high‐performance liquid chromatography
Author(s) -
Hu Kai,
Deng Zhifen,
Wang Bei,
Cui Yongxia,
Miao Mingsan,
Liu Wei,
Jiang Qiong,
Zhao Wenjie,
Huang Yanjie,
Zhang Shusheng
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400836
Subject(s) - alkylbenzenes , cyclophane , chemistry , calixarene , thermogravimetric analysis , phase (matter) , solvation , chromatography , reversed phase chromatography , reagent , triazine , organic chemistry , molecule , benzene
A new stationary phase for high‐performance liquid chromatography was prepared by covalently bonding a heteroatom‐bridged cyclophane onto silica gel using 3‐aminopropyltriethoxysilane as the coupling reagent. The structure of the new material was characterized by infrared spectroscopy, elemental analysis, and thermogravimetric analysis. The linear solvation energy relationship method was successfully employed to evaluate the new phase with a set of 25 solutes, and compared with octadecylsilyl and p ‐ tert ‐butyl‐calix[4]arene bonded stationary phases. The retention characteristics of the new phase are similar to the octadecylsilyl and conventional calixarene phases, and it also has distinctive features. In addition, the chromatographic behavior of the phase was illustrated by eluting alkylbenzenes and inorganic anions in the reversed‐phase mode and anion‐exchange mode, respectively. Thus, multi‐interaction mechanisms and mixed‐mode separation of the new phase can very likely guarantee its promising application in the analysis of complex samples. The column has been successfully employed for the analysis of triazines in milk, and it is demonstrated to be a competitive alternative analytical method for the determination of triazine herbicide residues.