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Design of guanidinium ionic liquid based microwave‐assisted extraction for the efficient extraction of Praeruptorin A from Radix peucedani
Author(s) -
Ding Xueqin,
Li Li,
Wang Yuzhi,
Chen Jing,
Huang Yanhua,
Xu Kaijia
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400828
Subject(s) - ionic liquid , extraction (chemistry) , chemistry , chromatography , organic chemistry , catalysis
A series of novel tetramethylguanidinium ionic liquids and hexaalkylguanidinium ionic liquids have been synthesized based on 1,1,3,3‐tetramethylguanidine. The structures of the ionic liquids were confirmed by 1 H NMR spectroscopy and mass spectrometry. A green guanidinium ionic liquid based microwave‐assisted extraction method has been developed with these guanidinium ionic liquids for the effective extraction of Praeruptorin A from Radix peucedani . After extraction, reversed‐phase high‐performance liquid chromatography with UV detection was employed for the analysis of Praeruptorin A. Several significant operating parameters were systematically optimized by single‐factor and L 9 (3 4 ) orthogonal array experiments. The amount of Praeruptorin A extracted by [1,1,3,3‐tetramethylguanidine]CH 2 CH(OH)COOH is the highest, reaching 11.05 ± 0.13 mg/g. Guanidinium ionic liquid based microwave‐assisted extraction presents unique advantages in Praeruptorin A extraction compared with guanidinium ionic liquid based maceration extraction, guanidinium ionic liquid based heat reflux extraction and guanidinium ionic liquid based ultrasound‐assisted extraction. The precision, stability, and repeatability of the process were investigated. The mechanisms of guanidinium ionic liquid based microwave‐assisted extraction were researched by scanning electron microscopy and IR spectroscopy. All the results show that guanidinium ionic liquid based microwave‐assisted extraction has a huge potential in the extraction of bioactive compounds from complex samples.