Premium
C 18 ‐bound porous silica monolith particles as a low‐cost high‐performance liquid chromatography stationary phase with an excellent chromatographic performance
Author(s) -
Ali Faiz,
Cheong Won Jo
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400811
Subject(s) - monolith , elution , chromatography , particle size , porosity , volumetric flow rate , trifluoroacetic acid , particle (ecology) , phase (matter) , resolution (logic) , high performance liquid chromatography , acetonitrile , chemistry , packed bed , silica gel , monolithic hplc column , materials science , analytical chemistry (journal) , catalysis , organic chemistry , physics , oceanography , quantum mechanics , artificial intelligence , computer science , geology
Ground porous silica monolith particles with an average particle size of 2.34 μm and large pores (363 Å) exhibiting excellent chromatographic performance have been synthesized on a relatively large scale by a sophisticated sol–gel procedure. The particle size distribution was rather broad, and the d (0.1)/ d (0.9) ratio was 0.14. The resultant silica monolith particles were chemically modified with chlorodimethyloctadecylsilane and end‐capped with a mixture of hexamethyldisilazane and chlorotrimethylsilane. Very good separation efficiency (185 000/m) and chromatographic resolution were achieved when the C 18 ‐bound phase was evaluated for a test mixture of five benzene derivatives after packing in a stainless‐steel column (1.0 mm × 150 mm). The optimized elution conditions were found to be 70:30 v/v acetonitrile/water with 0.1% trifluoroacetic acid at a flow rate of 25 μL/min. The column was also evaluated for fast analysis at a flow rate of 100 μL/min, and all the five analytes were eluted within 3.5 min with reasonable efficiency (ca. 60 000/m) and resolution. The strategy of using particles with reduced particle size and large pores (363 Å) combined with C 18 modification in addition to partial‐monolithic architecture has resulted in a useful stationary phase (C 18 ‐bound silica monolith particles) of low production cost showing excellent chromatographic performance.