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Preparation of two flavonoid glycosides with unique structures from barley seedlings by membrane separation technology and preparative high‐performance liquid chromatography
Author(s) -
Chen Tao,
Li Hongmei,
Zou DengLang,
Du YuZhi,
Shen YuHu,
Li Yulin
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400798
Subject(s) - chromatography , glycoside , flavonoid , chemistry , high performance liquid chromatography , membrane , organic chemistry , biochemistry , antioxidant
Barley seedlings are rich in flavones that can have positive effects on people with antihypoxia and antifatigue. Lutonarin and saponarin are two major flavonoid glycosides that have unique structures in barley seedlings. This study presents a new approach for the preparation of lutonarin and saponarin from barely seedlings by membrane separation technology and preparative high‐performance liquid chromatography. Preparative conditions of these two flavonoid glycosides by membrane separation technology were studied using response surface methodology. Under the optimized conditions, the total contents of these two flavonoid glycosides amounts to 17.0%.