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Online background cleanup followed by high‐performance liquid chromatography with tandem mass spectrometry for the analysis of perfluorinated compounds in human blood
Author(s) -
Liu Liangpo,
She Jianwen,
Zhang Xueqin,
Zhang Jie,
Tian Meiping,
Huang Qingyu,
Shah Eqani Syed Ali Musstjab Akber,
Shen Heqing
Publication year - 2015
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400761
Subject(s) - chromatography , chemistry , detection limit , tandem mass spectrometry , mass spectrometry , liquid chromatography–mass spectrometry , perfluorooctanoic acid , solid phase extraction , contamination , analytical chemistry (journal) , environmental chemistry , ecology , biology
In this study, a novel method for the analysis of perfluorinated compounds in whole blood has been developed and validated. The method was developed by using a conventional reversed‐phase C 18 column as a trapping column for the elimination of background contamination and high‐performance liquid chromatography with tandem mass spectrometry for the target compounds analysis. The trapping column provided fast online separation of the background contamination of perfluorinated compounds. In this developed method, the limits of detection for different perfluorinated compounds ranged from 0.06 to 0.14 ng/mL. It is notable that the limit of detection (0.07 ng/mL) for perfluorooctanoic acid was improved significantly after the elimination of background contamination. The method was also validated in terms of sensitivity, accuracy, and precision. The recoveries ranged from 66.8 to 111.9%, with relative standard deviations from 2.1 to 15.3%. Our preliminary data suggest that the novel method based on trapping column cleanup followed by high‐performance liquid chromatography with tandem mass spectrometry could be applied in studies on the human exposure to perfluorinated compounds.