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Kinetic approach for the purification of nucleotides with magnetic separation
Author(s) -
Tural Servet,
Tural Bilsen,
Ece Mehmet Şakir,
Yetkin Evren,
Özkan Necati
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400648
Subject(s) - nicotinamide adenine dinucleotide , adsorption , chemistry , langmuir adsorption model , magnetic nanoparticles , desorption , nicotinamide , chromatography , nanoparticle , nad+ kinase , organic chemistry , nanotechnology , materials science , enzyme
The isolation of β‐nicotinamide adenine dinucleotide is of great importance since it is widely used in different scientific and technologic fields such as biofuel cells, sensor technology, and hydrogen production. In order to isolate β‐nicotinamide adenine dinucleotide, first 3‐aminophenyboronic acid functionalized magnetic nanoparticles were prepared to serve as a magnetic solid support and subsequently they were used for reversible adsorption/desorption of β‐nicotinamide adenine dinucleotide in a batch fashion. The loading capacity of the 3‐aminophenyboronic acid functionalized nanoparticles for β‐nicotinamide adenine dinucleotide adsorption was 13.0 μmol/g. Adsorption kinetic and isotherm studies showed that the adsorption process followed a pseudo‐second‐order kinetic model and the experimental data can be represented using Langmuir isotherm model. The 3‐aminophenyboronic acid functionalized magnetic nanoparticles were proposed as an alternative support for the β‐nicotinamide adenine dinucleotide purification. The results elucidated the significance of magnetic separation as a fast, relatively simple, and low‐cost technique. Furthermore, the magnetic supports can be reused at least five times for purification processes.