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Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites
Author(s) -
Pelcová Marta,
Langmajerová Monika,
Cvingráfová Eliška,
Juřica Jan,
Glatz Zdeněk
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400582
Subject(s) - capillary electrophoresis , chromatography , repeatability , chemistry , dextrorphan , dextromethorphan , analyte , electrolyte , electrophoresis , capillary action , ammonium acetate , analytical chemistry (journal) , high performance liquid chromatography , electrode , materials science , medicine , biochemistry , receptor , anesthesia , composite material
This study deals with the nonaqueous capillary electrophoretic separation of dextromethorphan and its metabolites using a methanolic background electrolyte. The optimization of separation conditions was performed in terms of the resolution of dextromethorphan and dextrorphan and the effect of separation temperature, voltage, and the characteristics of the background electrolyte were studied. Complete separation of all analytes was achieved in 40 mM ammonium acetate dissolved in methanol. Hydrodynamic injection was performed at 3 kPa for 4 s. The separation voltage was 20 kV accompanied by a low electric current. The ultraviolet detection was performed at 214 nm, the temperature of the capillary was 25°C. These conditions enabled the separation of four analytes plus the internal standard within 9 min. Further, the developed method was validated in terms of linearity, sensitivity, and repeatability. Rat liver perfusate samples were subjected to the nonaqueous capillary electrophoretic method to illustrate its applicability.