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Simultaneous determination of nine lignans from Schisandra chinensis extract using ultra‐performance liquid chromatography with tandem mass spectrometry in rat plasma, urine, and gastrointestinal tract samples: Application to the pharmacokinetic study of Schisandra chinensis
Author(s) -
Kim YouJin,
Lee Hee Ju,
Kim Chul Young,
Han SunYoung,
Chin YoungWon,
Choi Young Hee
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400451
Subject(s) - schisandra chinensis , pharmacokinetics , chromatography , chemistry , lignan , schisandra , liquid chromatography–mass spectrometry , high performance liquid chromatography , tandem mass spectrometry , mass spectrometry , pharmacology , medicine , traditional chinese medicine , stereochemistry , alternative medicine , pathology
The fruit of Schisandra chinensis is a well‐known herbal medicine and dietary supplement due to a variety of biological activities including antihepatotoxic and antihyperlipidemic activities. However, the simultaneous validation methodology and pharmacokinetic investigation of nine lignans of S. chinensis extract in biological samples have not been proved yet. Thus, the present study was undertaken to develop the proper sample preparation method and simultaneous analytical method of schisandrol A, gomisin J, schisandrol B, tigloylgomisin H, angeloylgomisin H, schisandrin A, schisandrin B, gomisin N, and schisandrin C in the hexane‐soluble extract of S. chinensis to apply for the pharmacokinetic study in rats. All intra‐ and interprecisions of nine lignans were below 13.7% and accuracies were 85.1–115% and it is enough to evaluate the pharmacokinetic parameters after both intravenous and oral administration of hexane‐soluble extract of S. chinensis to rats.