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Rapid analysis of three β‐agonist residues in food of animal origin by automated on‐line solid‐phase extraction coupled to liquid chromatography and tandem mass spectrometry
Author(s) -
Mi Jiebo,
Li Shujing,
Xu Hong,
Liang Wei,
Sun Tao
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400442
Subject(s) - chromatography , chemistry , solid phase extraction , ractopamine , formic acid , extraction (chemistry) , electrospray ionization , mass spectrometry , tandem mass spectrometry , analyte , liquid chromatography–mass spectrometry , clenbuterol , detection limit , electrospray , sample preparation , selected reaction monitoring
An automated online solid‐phase extraction with liquid chromatography and tandem mass spectrometry method was developed and validated for the detection of clenbuterol, salbutamol, and ractopamine in food of animal origin. The samples from the food matrix were pretreated with an online solid‐phase extraction cartridge by Oasis MCX for <5 min after acid hydrolysis for 30 min. The peak focusing mode was used to elute the target compounds directly onto a C 18 column. Chromatographic separation was achieved under gradient conditions using a mobile phase composed of acetonitrile/0.1% formic acid in aqueous solution. Each analyte was detected in two multiple reaction monitoring transitions via an electrospray ionization source in a positive mode. The relative standard deviations ranged from 2.6 to 10.5%, and recovery was between 76.7 and 107.2% at all quality control levels. The limits of quantification of three β‐agonists were in the range of 0.024–0.29 μg/kg in pork, sausage, and milk powder, respectively. This newly developed method offers high sensitivity and minimum sample pretreatment for the high‐throughput analysis of β‐agonist residues.